cessation of SE, rats were given 5 ml intraperitoneal injection of 0.9% saline for hydration and treated with sufficient moistened pellets soaked in Gatorade. Control rats received lithium chloride, methylscopolamine-bromide and the same amount of 0.9% saline instead of pilocarpine. The pilocarpine-treated rats for the chronic period experiments were monitored by video recording (12 h/day for 2 days) to verify the emergence of spontaneous recurrent seizures. Acute, subacute, chronic phase in pilocarpine-treated rats started at 1, 7, and 21 days, respectively. All experiments were approved by the Institutional Animal Care and Use Committee at Seoul National University Hospital (IACUC # 13-0224). Methods were carried out in accordance with the approved guidelines. (n ≥ 3; unpaired t-test). Quantitative Real-time PCR assay: qRT-PCRs were conducted in triplicate using SYBR RT-PCR kit (Takara Bio Inc., Shiga, Japan) in an ABI 7500 (Life Technologies, CA, USA). The qRT-PCR reactions were carried out at 95 °C for 30 s, and 35 cycles of 95 °C for 5 s and 60 °C for 34 s. PCR primers for NEAT1 were 5′-GTTCCGTGCTTCCTCTTCTG-3′ (forward) and 5′-GTGTCCTCCGACTTTACCAG-3′ (reverse). β-actin primers (forward: 5′-AAGACCTCTATGCCAACACAGT-3′, 5′-reverse: GCTCAGTAACAGTCCGCCTA-3′) were used for RNA normalization. (2) Kainic acid (KA)-induced epilepsy rat model:
