in vitro and in vivo when they were transplanted into the 6-hydroxydopamine lesioned striatum model of Parkinson’s disease and showed electrophysiological properties similar to that of primary dopaminergic neurons. While this optimized protocol yielded iDaN cells that are able to restore dopamine function in vivo, the gene expression profile of key midbrain dopamine marker genes was still different from endogenous Pitx3-EGFP midbrain dopamine neurons and the degree of functional restoration was significantly lower than when primary dopamine neurons were transplanted.
