representation of transcriptome data in native anatomical coordinates. Figure 1e illustrates the specificity of anatomical sampling using this representation. For example, the folate receptor FOLR1 is selectively expressed in VZ and GE. Sufficient folate intake is essential for proper neuronal development,26 and mutations in FOLR1 cause severe neurological sequelae due to cerebral folate transport deficiency. Similarly, two genes associated with abnormal cortical development in holoprosencephaly, TGIF1 and SIX327, are also enriched in cortical germinal zones. Finally, structural magnetic resonance imaging (MRI) and diffusion weighted MRI (DWI) data from 3 approximately age-matched brains (Fig. 1c; Extended Data Fig. 2), as well as reconstruction of fetal white matter tracts for three additional brains (Extended Data Fig. 3; see28), are included for anatomical reference in the online resource.
