= 20 vs. 0.85 ± 0.07, n = 18; p = 0.16) in oocytes injected with the presumed native 2:2:1 ratio of α2β2γ3 subunits. Likewise, chronic ethanol (44 mM) did not significantly change, although a trend is apparent, GABA currents in oocytes injected with a 6:2:1 ratio of subunits (1.00 ± 0.19, n = 14 vs. 0.52 ± 0.18, n = 8; p = 0.11), and had no effect on oocytes injected with a 0.5:2:1 ratio (1.00 ± 0.19, n = 12 vs. 1.06 ± 0.18, n = 17; p=0.84). In contrast, 100 mM ethanol inhibited GABA currents by 25% (1.0 ± 0.11, n = 23 vs. 0.75 ± 0.0.07, n = 28, p < 0.05) in oocytes injected with the presumed native 2:2:1 ratio of α2β2γ3 subunits (Figure 4B). Chronic ethanol also reduced GABA currents in oocytes injected with a 6:2:1 ratio of subunits (1.00 ± 0.07, n = 38 vs. 0.76 ± 0.08, n = 43, p < 0.05), but had no effect on oocytes injected with a 0.5:2:1 ratio (1.00 ± 0.06, n = 23 vs. 0.92 ± 0.08, n = 28; p = 0.42).
