Individually-housed mice were video recorded in 12″ × 12″ × 13.5″ acrylic chambers, using infra-red cameras during the dark phase, for 72 continuous hours under 12/12 light/dark conditions. The distance traveled in meters per hour was calculated by Noldus Ethovision software, and averaged across 8 CTRL mice (gray fill, lines 22 and 17) and 10 SCZ mice (purple fill, line 52). In addition, transitions between phases of the light cycle (measured 30 minutes before and 30 minutes after light changes) were analyzed in terms of the number of consecutive seconds of immobility as a percentage of total immobility (AnyMaze, Stoelting), per 30 min measurement block as described (McShane et al., 2010; Pack et al., 2007).
