the extraction of marker genes from the reference samples (function extractMarkers from the CellMix package), and the posterior invocation of the function ged to infer cellular population from the gene expression of bulk RNA-seq data. Besides, we tested additional methods which provided considerably lower accuracy (least-squares fit [58], quadratic programing [59]) or no significant difference (support vector regression [26] or latent variable analysis [60]) to the methods presented.
