Fig. S1 tests BK channel kinetics at different voltages in response to [Ca2+] jumps between 1 and 320 µM. Fig. S2 compares the effects of ICa(spark) amplitude on simulated and measured STOCs. Fig. S3 examines the voltage dependence of Po(t) of BK channels from the puncta marked in Fig. 5 A. Video 1 shows the 3-D distribution of RYR3 and BK channels. Video 2 shows the 3-D distribution of RYR1. Video 3 shows the 3-D distribution of RYR2. Video 4 shows the spatial relationship between RYR2s and BK channels. Video 5 displays the evolution of a Ca2+ spark acquired at 333 Hz. Online supplemental material is available at http://www.jgp.org/cgi/content/full/jgp.201110626/DC1.
