The ErGeN3 member with the highest degree of connectivity was a probe-set (1435583_at) annotated as AU067633. However, recent data from RNA-Seq analysis of B6 and D2 brain transcripts (Lu and Williams, personal communication) strongly suggests that this probe-set actually targets the distal 3′ untranslated region of Grm3, a metabotropic glutamate receptor (Figure S5). Given the considerable evidence that metabotropic glutamate receptors are key mediators of the neuroadaptations associated with addiction [52], Grm3's position as a major hub of this ethanol-responsive network has mechanistic implications for regulation of the network and further supports the overall significance of this network in ethanol traits.
