The deletion of the extended ARID was also engineered in the Dri.SSS background. The resulting mutant is designated Dri.SSS.ΔC. In the DNA pull-down assay, the Dri.SSS.ΔC construct (Figure 8) has the same reduced DNA-binding affinity as was seen in the Dri.SSS mutant in Figure 6B. The sequence selectivity is further reduced, but a weak selectivity is still evident.
