(136 kDa; Figure 3a). For our ChIP assays 1 µg of DNA was immunoprecipitated with anti-myc-tag antibody or negative control IgG and expressed as a percentage of input. Chromatin immunoprecipitated for ZNF804a-myc was enriched in single distinct peaks within the PRSS16 (Figure 3b; 26.2±0.4%, p<0.05; n = 4) and COMT (Figure 3c; 20.2±0.2%, p<0.05; n = 4) 2 kB regions, but not within 2 kB upstream of PDE4B or DRD2 genes (Figure 3d). Furthermore, the genomic fragments that immunoprecipitated with ZNF804a contained predicted zinc-finger interacting motifs (COMT GGCGG; PRSS16 GGCG). These results indicate that overexpressed ZNF804a directly interacts with the promoter regions of PRSS16 and COMT and is consistent with ZNF804a having a direct positive effect on the transcription of these two SZ associated genes.
