cortical microglia; the membrane distribution accentuates the finer extended processes (Figure 7B–D)(Baron et al., 2014). Tmem119 and Iba1 were also expressed in both hCyto+ soma and in highly arborized iMGL processes (Figure 7E–L). At higher magnification, Tmem119 is predominately membrane-bound and in agreement with previous studies (Bennett et al., 2016). Together, these findings demonstrate engraftment and long-term survival of iMGLs that result in highly branched microglia-like cells expressing Iba1, P2ry12 and Tmem119 (Figure 7I–L), in which iMGLs resemble endogenous quiescent microglia. Finally, the morphology and high P2ry12 expression suggest that transplanted iMGLs are actively surveying their neuronal environment that translates to their potential use in studying human microglia function in mouse CNS-disease models.
