We found high correlations between visits for each method (ρ = 0.96-0.99) but lower correlations between different methods (ρ = 0.79-0.98) suggesting that the cell or RNA isolation method has a larger impact on the gene expression profile than the variability between visits. The decreased correlations between LCLs and B-cell CD19 or B-cell CD20 might have resulted from the controlled in vitro conditions of the LCLs or the B-cell purifications.