Using Bioconductor requires a willingness to modify and eventually compose scripts in a high-level computer language, to make informed choices between different algorithms and software packages, and to learn enough R to do the unavoidable data wrangling and troubleshooting. Alternative and complementary tools exist; in particular, users may be ready to trade some loss of flexibility, automation or functionality for simpler interaction with the software, such as by running single-purpose tools or using a point-and-click interface.
