The single SNP results for MDD are depicted in Figure 3 and Table 4a. Our analytic plan dictated the combined analysis of all replication samples with the use of a one-tailed directional test. No association in the replication sample reached statistical significance after correction for multiple comparisons and SNP non-independence due to LD (ninth column in Table 4a). Similarly, haplotype analyses did not reveal significantly associated regions (Supplemental Figure 16). There were four p-values <0.05 in the replication sample but only rs10954694 also had Z-scores of the same sign in both samples. Table 4b shows the results for reoMDD, and no single SNP was significant after correction for multiple comparisons. When we repeated the MDD analyses restricted to female subjects, the observed significance levels did not become markedly stronger in any of the replication samples in contrast to the initial NESDA/NTR sample. Thus, results from analyses of all replication samples did not reach the a priori criterion for replication evidence for the involvement of PCLO in the etiology of MDD.
