targeted bisulfite sequencing is available. For example, Agilent uses a large number of bead-immobilized hybridization probes to enrich CpG islands, promoters, and related sequence as part of its SureSelect™ Methyl-Seq Enrichment System. In summary, the microarray-based methods have the advantage of being less expensive to run and simpler to interpret. The use of antibodies to immunoprecipitate methylated C residues is likely to be less sensitive than direct bisulfite sequencing. Sequencing has the advantage of single-base resolution but makes data analysis much more challenging. Many of these methods have been used to study ethanol-induced regulation of DNA methylation.
