(approximately 12 MB) due to memory limitations. iNPS differs from other nucleosome callers in that it uses the wave-like structure of nucleosome datasets as part of its smoothing approach. iNPS detects nucleosomes with various shapes from the first derivative of the Gaussian smoothed profile. DANPOS differs from all above approaches in that it allows the comparison of MNase-seq datasets and identifies dynamic nucleosomes based on fuzziness change, occupancy change and position shift. In addition, DANPOS performs well in assigning nucleosomes from a single experiment, and should prove an invaluable analysis tools for deciphering underlying chromatin perturbations responsible for various disease and cellular phenotypes.
