To address this question, alcohol and saccharin exposed 3xTg-AD and WT controls were tested for PPI of the startle response. Results showed that saccharin exposed 3xTg-AD mice exhibited normal PPI, but a history of alcohol drinking was associated with a pronounced and significant impairment in PPI across a range of stimulus intensities (Fig. 5B). Three-way RM-ANOVA (alcohol × genotype × stimulus intensity, dB) with identified a significant main effect of both genotype [F(1, 12) = 6.777, P = 0.023] and dB [F(3, 36) = 44.7, P < 0.0001], and a significant alcohol × dB interaction [F(1, 13) = 4.527, P = 0.038].
