Studies were conducted in drug-naïve C57BL/6 J (B6), FVB/NJ (FVB), NZB/B1NJ (NZB), and F1 hybrid mice derived from these three progenitors (FVBxB6 F1, maternal strain × paternal strain; B6xNZB F1). B6, FVB, and NZB breeders as well as Cd14 (B6.129S-cd14tm1Frm/J; Stock #003726) null mice were purchased from Jackson Laboratories (Bar Harbor, ME) and mated at the age of 8 weeks in the Texas Genetic Animal Core of the Integrated Neuroscience Initiative on Alcohol (INIA) at the University of Texas at Austin. Cd14 knockout mice were backcrossed on C57Bl/6 J genetic back- ground more than 10 times. Mice (four–five per cage) were housed in standard polycarbonate shoebox cages with food (Prolab RMH 1800 5LL2 chow) and water provided ad libitum. The colony rooms and testing rooms were maintained at an ambient temperature of 21 ± 1 °C, humidity (40–60%), and centrally controlled ventilation (12–15 cycles/h with 100% exhaust). Colony rooms were on a 12:12 light/dark light cycle (lights on at 07:00 AM). All procedures were approved by the Institutional Animal Care and Use Committee and adhered to NIH Guidelines. The University of Texas facility is AAALAC accredited.
