Ethanol consumption was measured in individually housed animals presented with a continuous choice between tap water and an ethanol solution. The duration of the procedure was 10 days. For the first 2 days, animals were presented with two water bottles, and on each day, the volume of water consumed from each bottle was measured. On day 3, 3% ethanol in water (v/v) was substituted for one of the water bottles. On day 7, 10% ethanol was substituted for the 3% ethanol solution. The position of the ethanol bottle was changed on days 5, 7, and 9. Ethanol consumption was measured as the average consumption on days 4 and 6 (3% ethanol) and on days 8 and 10 (10% ethanol). Data are reported as grams per kilogram ethanol consumed. The consumption of 10% ethanol was the selection phenotype. Data were also collected for total fluid volume consumed, which were used to measure ethanol preference.
