To probe potential mechanisms or gene/protein/phosphorylated protein networks related to the functional differences observed in our electrophysiology experiments, a multi-omic analysis of the DS in HAP and LAP mice was employed. Minimal overlap was observed in the transcriptome and proteome results which is commonly reported across numerous tissues and cell types (Chen et al. 2002; Pascal et al. 2008; Ghazalpour et al. 2011; Chen et al. 2019; Hausser et al. 2019). This discordance between mRNA and protein abundance is likely a result of post-transcriptional/post-translational processing and mRNA vs protein degradation rates. (Vogel & Marcotte 2012). Additionally, as mRNA transcripts are mostly located in cell bodies, the RNA-sequencing may not fully capture line differences present in the dense presynaptic inputs from other brain regions that project to the DS. However, proteomic and phosphoproteomic analyses possess a greater capacity to discover line differences in protein expression from both neurons of the DS and presynaptic inputs arriving from other brain regions. Alternatively, proteomics is less sensitive to lowly expressed proteins (as one cannot amplify proteins similar to amplifying mRNA), and additional biological replicates
