ketamine/xylazine and perfused transcardially with 0.9% saline followed by 4% paraformaldehyde. The brain samples were postfixed with 4% paraformaldehyde and equilibrated in 30% sucrose. Coronal sections of 40 μm were prepared with a sliding microtome. Brain sections of one-in-four series were selected for immunostaining. Neurons in the granular layer of the DG were traced on an upright stereoinvestigator microscope (maker) under 40× objective and morphometric analyses were done using Neurolucida (MBF Bioscience).
