To assess the validity of our data, we conducted two proof-of-principle GWAS. The first trait we studied was BMI, and in line with what expected, we observed strong evidence of associations with known BMI loci including FTO and TMEM18 that both reached genome-wide significance (P<5x10-8). In addition, out of 32 known BMI SNPs we observed nominal significance (P<0.05) for 19 of them, all in the same direction as expected from previous reports. Of note, our sample size (n = 20,823) is less than 10% of the original GWAS that had a total sample size of 249,766 individuals. Therefore, we would not expect to observe significant associations for all BMI SNPs due to limited power. For VTE, we observed genome-wide significant associations for the F5 and ABO loci that are both known to be associated with VTE. In addition, we also observed a nominal association (P = 0.007) with the F11 region. Our BMI and VTE results confirm that GWAS analysis of secondary traits in this data is valid and provides a platform for future studies of secondary traits. We ran
