Consistent with several prior studies 6, rs1229984 in ADH1B was associated with DSM-IV AD. Although GWS was only noted in the trans-ancestral (EA+AA) analysis, as shown in Supplemental Table 7, rs1229984 was associated with the AD criterion count and criteria indexing physiological dependence and Desire to cut drinking at GWS levels, and with other AD criteria at nominal levels of significance. Despite the robust relationship between this functional variant and AD, its relatively low minor allele frequency necessitates fairly large samples to detect a GWS effect for a binary trait, as was shown in a recent meta-analysis of DSM-IV AD 6. However, for DSM-IV AD criterion count, rs1229984 was GWS in both the EA and EA+AA analyses. Similar to another study 16, we found that while rs1229984 was associated with each individual criterion (EA all p<3.61E-04; EA+AA all p<4.54E-05), the association was stronger with certain DSM-IV AD criteria. Consistent with Hart et al., Tolerance was strongly associated with rs1229984 (p=8.06E-09 in EA+AA). However, the additional GWS associations with Desire to cut drinking in our study differs from the prior study
