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Chunk #30 — Methods — Benchmark dataset

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Statistical modeling for sensitive detection of low-frequency single nucleotide variants.
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The length of targeted regions for Illumina MiSeq datasets is 23.2 kb, covered by 158 amplicons. The design details can be found in the paper [15] and Additional file 3. The raw reads were downloaded from NCBI Short Read Archive (SRP009487.1) and processed as the paper described. Reads with mapping quality less than 30 were filtered out.