Levels of 24,526 transcripts for 18,401 genes were measured in 773 brain samples from the cerebellum and temporal cortex of ∼200 ADs and ∼200 non–ADs, using WG-DASL assays. Nearly 70% of all probes could be detected in >75% of the samples tested. All autopsied subjects were genotyped for 313,330 single nucleotide polymorphisms (SNPs) from Illumina HumanHap300-Duo Genotyping BeadChips, as part of the Mayo AD GWAS [29]. An eGWAS testing association of transcript levels with cisSNPs was performed using multivariable linear regression correcting for APOE ε4 dosage, age at death, gender and multiple technical variables. False discovery rate (FDR)-based q values [30] (q) were used for corrections of multiple testing.
