15–75 μg of protein were resolved on 12% Bis-Tris or 4–12% gradient Bis/Tris gels (Life Technologies) and the proteins were transferred to nylon membranes (Bio-Rad). For Aβ WB analysis, the membranes were cross-linked with 0.5% glutaraldehyde solution before blocking. WB images were visualized by enhanced chemiluminescence (ECL). The images were captured by using BioMax film (Kodak, Rochester, NY, USA) or VersaDoc imaging system (Bio-Rad) and quantitated by using QuantityOne software (Bio-Rad). Primary antibodies were used at the following dilutions: 6E10 anti-Aβ (1:300, Convance), anti-PS1 (1:1,000, Cell Signaling Technology), anti-a-tubulin (1:1,000, Cell Signaling Technology), anti-CNPase (1:1,000, Cell Signaling Technology) and anti-BACE1 (1:1,000, Cell Signaling Technology), C66 APP C-terminal antibody (1:2000), AT8 anti-p-tau (1:100, Millipore), PHF1 anti-p-tau (1:500), anti-total tau (1:2,000, DAKO), anti-MAP2 (1:500, Millipore; 1:200, Cell Signaling), anti-NCAM (1:1000, Cell Signaling), anti-synapsin I (1:500, Cell Signaling), anti-HSP70 (1:1000, Enzo Life Sciences, Farmingdale, NY, USA) and anti-human mitochondrial antigen (h-mito, 1:500, Millipore).
