miPSCs were differentiated into neurons using a similar protocol as described above. This culture condition generated 92 ± 3% MAP2+ neurons, including 74 ± 4% vGLUT+ total glutamatergic neurons, 32 ± 4% Tbr1+ subclass of glutamatergic neurons, 13 ± 3% GABA+ GABAergic neurons (for apoE3/3-miPSCs; 5 ± 2% for apoE4/4-miPSCs), and 5 ± 2% TH+ dopaminergic neurons. In this neuronal culture, GFAP+ astrocytes were 3 ± 1%. The percentages of these different cell types generated from miPSCs were very similar to those generated from hiPSCs.
