iPS cells were differentiated into neural cells using an established protocol (#SOP-CH-207 Rev A) developed by the WiCell Institute (www.wicell.org, Madison, WI, USA) for the neural differentiation of hES cells. Confluent iPS colonies were harvested from 10 cM plates using 1mg/mL dispase in DMEM/F12. iPS cells were cultured in suspension for 4 days in 75mL low-adhesion flasks using hES cell media without the addition of bFGF to allow for the formation of embryoid bodies (EBs). Half of the media was changed daily. After 4 days, EBs were collected, centrifuged, and washed in phosphate buffered saline (PBS) and placed into new 75mL low adhesion flasks in a neural induction media [DMEM/F12 supplemented with 1× N2 supplement (Invitrogen), 1× non-essential amino acids and 2µg/mL heparin (Sigma-Aldrich, St. Louis, MO, USA)] to allow for the formation of neural epithelial (NE) cells. Half of the media was changed every other day. After 2–4 days in suspension, NE cells were collected and plated onto laminin (20µg/mL in DMEM/F12) coated 6-well cell culture plates to allow the NE cells to adhere to the surface. Cells were
