To understand the possible cause that rendered reduced efficacy of BACE1 inhibitor in 3D neuronal culture derived from the subject AD1, we prepared lysates from 3D neurons and subjected them to proteomic analysis using Mass Spectrometry. We analyzed these samples by labelling tryptic peptides with TMT 6-plex reagents. The relative levels of several gene products were calculated; since the efficacy of BACE1 inhibitor was reduced in AD1, we compared individual subjects to subject AD1 (Table 2).
