clone ID: HsCD00413295), of MBIP for inhibiting DLK (pLX304-MBIP; HsCD00420627), and of MKK7 (pCW45-MKK7; HsCD00298961); for all lentiviral plasmids were obtained from Harvard Medical School; iii, Lentiviruses for overexpression of dominant-negative cFos (DN cFos, Olive et al., 1997)(cDNA from Addgene plasmid # 33353 and cloned into AgeI and EcoRI sites on lentiviral vector FUGW Addgene plasmid # 14883); and iv. Lentiviruses for shRNA-mediated DLK or LZK knockdown, for CRISPR-mediated inhibition of MKK7 expression, and for CRISPRi of the APP promoter as described in details below.
