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Chunk #14 — Methods — Genotyping and genotype grouping

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Association between dopaminergic polymorphisms and borderline personality traits among at-risk young adults and psychiatric inpatients.
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In the genetic analyses the VNTRs were analyzed according to the number of the risk allele, i.e. the DRD4 48 bp VNTR 7-repeat allele and the DAT1 40 bp VNTR 9-repeat allele (see Table 2 and 3). In the second set of analyses the genotypes were grouped according to the presence of the risk allele, resulting in DRD4 7-present (7+) and 7-absent (7-) groups. At the DAT1 VNTR the grouping was based on the presence of the 9-repeat allele, resulting in 9-present (9+) and 9-absent (9-) groups; the rare 3/10, 6/10, 10/11, and 10/12 genotype were grouped together with the 10/10 genotype in the 9- group, whereas the rare 3/9 and 8/9 genotype were regarded as having one 9-repeat allele, and grouped to the 9+ group. At the SNP markers, homozygotes for the minor allele were grouped together with heterozygotes in the second round of analyses, i.e. DRD2 A1+ group consisted of A1/A1 (TT) and A1/A2 (CT) genotypes, DRD2 B+ group consisted of B1/B1 (AA) and B1/B2 (AG) genotypes. The DRD4 -521 C/T SNP was analyzed in CC + CT vs. TT setting, and at the -616 C/G SNP CC vs. CG + GG groups were compared.