Dorsolateral Prefrontal Cortex: Total RNA was isolated from approximately 100 mg homogenized tissue in Trizol using the RNeasy kit according to manufacturer’s protocol and processed together in treatment and sex randomized batches of 12 samples. The mean total RNA yield was 28.5 ug. The RNA Integrity Number (RIN) was determined by fractionating RNA samples on the 6000 Nano chip (Agilent Technologies) on the Agilent 2100 Bioanalyzer. Samples with RIN > =5.5 were included in the sequencing set, resulting in 2 samples with RIN < 5.5 being excluded from the study. Among the remaining samples the mean RIN was 7.3 and the mean ratio of 260/280 was 2.1.
