Non-neuronal cells exhibited broadly consistent expression signals across brain regions. To better appreciate diversity among non-neuronal classes, we grouped single-cell libraries across regions by cell class and performed semi-supervised ICA on each of the seven non-neuronal datasets independently, identifying a total of 53 biological ICs (Figure 2A and Data S4A–G). We focus here on cell classes that form the blood-brain barrier – mural, fibroblast-like and endothelial cells – because they are disease-relevant (Sweeney et al., 2016) and remain incompletely characterized (Figure 2B).
