Intronic DNA methylation is reported to be involved in regulating alternative splicing [27,28]. Although, it is known that Mecp2 isoforms are generated by alternative splicing [4,5], the underlying molecular mechanisms are still unclear. We observed that the expression ratio of Mecp2e1/Mecp2e2 changed during NSC differentiation. The observed correlation between the splice ratio and intron 1 R4 DNA methylation in differentiating NSC at D2 would provide insights on the potential importance of this region in Mecp2 alternative splicing.
