HES-3 NKX2-1GFP/w and H1 human ES cells, and human iPSC (1090) were maintained in feeder-free culture condition. iPSC line 1090 was derived from peripheral blood mononuclear cell of a male donor by overexpressing the reprogramming factors OCT4, SOX2, KLF4, and MYC through Sendai virus vectors, and the pluripotency was confirmed (Figure S5A and S5B). hPSCs were cultured on Matrigel coated cell culture dish with mTeSR1 media, and were passaged every 7 days by Dispase (0.83 U/ml) treatment.
