Detection of chromatin accessibility genome-wide with all the above methods requires initial library construction and use of NGS [31, 104]. Resulting data represents an average in vivo snapshot of chromatin accessibility, as represented in the constructed sequencing libraries. Normally, a specialized sequencing facility performs library construction and sequencing using the appropriate kits for the operated sequencer. Otherwise, a research laboratory can use in-house instrumentation and manufacturer or custom library protocols, with the latter being more cost efficient.
