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Chunk #6 — Methods — SNP Selection and Genotyping

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Association of blood lipids with common DNA sequence variants at 19 genetic loci in the multiethnic United States National Health and Nutrition Examination Survey III.
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Genotyping was performed on the Sequenom platform, which utilizes matrix-assisted laser-desorption ionization time-of-flight mass spectroscopy as described previously.18 Using 356 samples in duplicate, we found the genotyping error to be 0.34%. SNP rs693 in the APOB gene was excluded from analysis due to a low genotyping call rate (88%). SNP rs10774708 near the MMAB/MVK genes was excluded from analysis due to a low genotyping call rate (70%). In sum, 19 SNPs from 17 loci were successfully genotyped and the genotyping success rate for each of these 19 SNPs exceeded 92%.