To further probe the migratory capacity of the day 2-18 versus 10-18 treated cells, we transplanted FACS-isolated NKX2.1::GFP+ cells into the neonatal neocortex of, genetically immuno-compromised mice. Four weeks after transplantation we observed extensive migration, in both the radial and tangential planes within the mouse cortex but only for the day 10-18 group (Figure 3O,P). Similar results were obtained upon transplantation into the adult cortex (Figure S2) though overall migration of NKX2.1::GFP+ cells by day 30 in vivo was less extensive compared to the neonatal grafts.
