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Chunk #15 — RESULTS — Experiment 1: The Effect of D-Lys3-GHRP-6 on preference to alcohol in a two-bottle choice experiment

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Ghrelin receptor antagonism decreases alcohol consumption and activation of perioculomotor urocortin-containing neurons.
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0.68 ± 0.04. On day 4, injection of D-Lys3-GHRP-6 blocked preference to ethanol (0.49 ± 0.06) [F(1,10) = 6.79, p = 0.026). Ethanol intakes also did not change over the first three experimental days. Intake on day 4 was 2.92 ± 0.17 g/kg, on day 5, 3.38 ± 0.21 g/kg and on day 6 was 3.18 ± 0.23 g/kg. D-Lys3-GHRP-6 showed a trend to decrease ethanol intake but this did not reach significance (p = 0.085) (Fig. 1B). Total volume of fluid consumed on day 4 was 1.08 ± 0.06 ml, on day 5, 1.01 ± 0.08 ml and on day 6, 0.97 ± 0.07 ml (no significant difference over the three days). There was no change in the total volume consumed between the group that was administered D-Lys3-GHRP-6 and the saline-treated group (Fig. 1C).