We performed 76–base pair (bp) paired-end mRNA sequencing on a total of 1749 samples, of which 1641 samples from 43 sites, and 175 donors, constituted our final “pilot data freeze” reported on here (14). Median sequencing depth was 82.1 million mapped reads per sample (fig. S3A). The final data freeze included samples from 43 body sites: 29 solid-organ tissues, 11 brain sub-regions (with two duplicated regions), a whole-blood sample, and two cell lines derived from donor blood [EBV-transformed lymphoblastoid cell lines (LCLs)] and skin samples (cultured fibroblasts) (Table 1 and tables S1 and S2). Median sample size for the nine high-priority tissues was 105; median sample size for the other 34 sampled sites was 18.5.
