We assessed cell-type specificity in the up- and down-regulated genes for each treatment by determining if there is an overrepresentation of cell-type specific genes in the regulated gene-sets (Figure 2). In the amygdala, each PPAR agonist regulated a large number of genes that are preferentially expressed in neurons (feno and tesa p < 0.002; beza p < 0.02) (Figure 2A). In order to classify potential targeted neurons, we used datasets of genes preferentially expressed in GABAergic and glutamatergic neurons. All treatments down-regulated glutamatergic genes, and feno and tesa (but not beza) up-regulated GABAergic genes in the amygdala (Figure 2A). There was not an overrepresentation of either neuronal subtype in the PFC (Figure 2b).
