Chunk #41 — Methods — Cellular differentiation assay (‘Cellomics’) — Differentiation potential assay

Source: Common genetic variation drives molecular heterogeneity in human iPSCs.
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For differentiation to neuroectoderm, iPSCs were grown for 12 days in CDM-PVA supplemented with SB431542 (10mM; Tocris Bioscience), FGF2 (12 ng/ml, CSCR University of Cambridge), and Noggin (150 ng/ml, R&D Systems Inc.). Media was changed daily.