Fig. 1), knockdown of TBC1D5 enhances the fluorescence intensity of VPS26 and the CIMPR. Fam21 staining in the VPS35 D620N-expressing cells was markedly reduced compared with levels in cells expressing wild-type VPS35. Loss of TBC1D5 expression enhances the Fam21 fluorescence in both wild-type and D620N-expressing cells, but notably, the increase in Fam21 staining in the cells expressing the VPS35 D620N mutant results in levels of Fam21 staining that are almost the same as cells expressing wild-type VPS35. The gain in intensity for Fam21 staining was found to be statistically significant and the P-values are shown in the legend for Fig. 4C.
