minutes at 4°C. The supernatant was discarded, 500 μl of cold 80% ethanol (Fisher, 5739852) wash buffer was added and the DNA pellet was spun down by centrifugation at the maximum speed for 5 minutes at 4°C. The pellet was air dried at room temperature for 20 minutes and re-suspended in 10 μl 10 mM Tris-HCL, pH 8.0 (Sigma St Louis MO, USA, T2694). Fragmented genomic DNA was analyzed by gel electrophoresis on a 0.8% agarose gel to confirm that the genomic DNA was in the correct size range (2 to 4 kb). To prepare the input DNA template mixture for targeted amplification, 1.5 μg of the purified genomic DNA fragmentation reaction was added to 4.7 μl 10× High-Fidelity Buffer (Invitrogen, 11304-029), 1.26 μl of MgSO4 (Invitrogen, 11304-029), 1.71 μl 10 mM dNTP (New England Biolabs, Ipswich MA, USA, NO447S/L), 3.6 μl Betaine (Sigma, B2629-50G), 3.6 μl of RDT Droplet Stabilizer (RainDance Technologies, 30-00826), 1.8 μl dimethyl sulfoxide (Sigma, D8418-50 ml) and 0.72 μl 5 units/μl of Platinum High-Fidelity Taq (Invitrogen, 11304-029) and the samples were brought to a final volume of 25 μl with nuclease free water (Teknova-Fisher Hollister CA, USA, 50843418).
