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Chunk #18 — 2 METHODS — 2.3 Model fitting and parameter estimation

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SNVMix: predicting single nucleotide variants from next-generation sequencing of tumors.
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We fit the model using the EM algorithm. We initialize μk and π(k) to their prior means. The EM algorithm iterates between the E-step where we assign the genotypes using Equation 2 and the M-step where we re-estimate the model parameters. At each iteration, we evaluate the complete data log-posterior and the algorithm terminates when this quantity no longer increases. The M-step equations are standard conjugate updating equations: (3) where I(Gi=k) is an indicator function to signal that Gi is assigned to state k at position i, and: (4)