Chunk #52 — Materials and Methods — Subcloning and strain construction

Source

Replicative age induces mitotic recombination in the ribosomal RNA gene cluster of Saccharomyces cerevisiae.

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yes

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The two-chromosome LOH reporter strain UCC8918 was generated by mating UCC8917 x UCC5181. UCC8917 was derived from UCC5179 by one-step integration of a PCR fragment carrying ADE2 into an intergenic region of chromosome IV (coordinates 1,515,634–1,515,738), which was generated using oligos MarthaN/H2L and MarthaN/H2R with pRS402 [63] as a template.