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Chunk #11 — METHODS — Receptor binding and autoradiography

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Social isolation and chronic handling alter endocannabinoid signaling and behavioral reactivity to context in adult rats.
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25 °C) and blown dry. Sections were apposed to [3H]-sensitive film (Amersham Hyperfilm, GE Healthcare LifeSciences, Piscataway, NJ) with [3H] standards ([3H] microscales, Amersham, Arlington Heights, IL, USA) for 8 weeks for levels incubated in 4.6 nM [3H]CP55,940 and 9 weeks for levels incubated in 3.3 nM [3H]CP55,940. Films were developed in D19 developer (Carestream Health, Rochester, NY, USA) and air-dried.