25 °C) and blown dry. Sections were apposed to [3H]-sensitive film (Amersham Hyperfilm, GE Healthcare LifeSciences, Piscataway, NJ) with [3H] standards ([3H] microscales, Amersham, Arlington Heights, IL, USA) for 8 weeks for levels incubated in 4.6 nM [3H]CP55,940 and 9 weeks for levels incubated in 3.3 nM [3H]CP55,940. Films were developed in D19 developer (Carestream Health, Rochester, NY, USA) and air-dried.