weeks of re-plating onto a monolayer of mouse glia. The iN cells of both genotypes exhibit similar intrinsic membrane properties (Supplemental Fig. 1D–F), can fire repetitive spontaneous action potentials (APs) at baseline levels (Supplemental Fig. 1G–I), and exhibit similar intrinsic excitability at baseline conditions (Fig. 1H–I). Similarly, no significant differences in spontaneous or miniature inhibitory postsynaptic currents (sIPSCs and mIPSCs, respectively) were observed by genotype (Figs. 1E–G, Supplemental Fig. 1J–L), indicating that the N40D variant does not affect passive or active membrane properties and that the neurons generated from subject iPS cell lines are of similar functional maturation and differentiation status.
