For these reasons, we explored feeding as a means of delivering dsRNA for RNAi. We have developed an optimized protocol for feeding which is of similar sensitivity to injection and results in phenotypes at least as strong as those produced by injection. Furthermore, the interference effect produced by feeding can be titrated, resulting in the ability to generate a range of strong and hypomorphic phenotypes analogous to an allelic series of mutants. Thus, this method establishes RNAi by feeding as a viable or even preferable alternative to RNAi by injection in C. elegans.
