Chunk #58 — Materials and Methods — Pedigree analysis

Source

Replicative age induces mitotic recombination in the ribosomal RNA gene cluster of Saccharomyces cerevisiae.

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Pedigree analysis was performed as previously described [11]. Briefly, daughter cells born to individual mothers were micromanipulated to new positions on a YEPD plate and allowed to form colonies. Colonies were scored for mitochondrial function by replica plating to YEP+glycerol.